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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
17/05/2016 |
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Data da última atualização: |
17/05/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, M. R. A. dos; GUIMARÃES, M. C. M.; PAZ, E. S.; MAGALHÃES, G. M. O.; SOUZA, C. A.; SMOZINSKI, C. V.; NOGUEIRA, W. O. |
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Afiliação: |
MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia; UNIR; UNIR; UNIR; Bolsista, Embrapa Rondônia; Bolsista, Embrapa Rondônia; UNIR. |
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Título: |
Induction and growth pattern of callus from Piper permucronatum leaves. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Revista Brasileira de Plantas Medicinais, Botucatu, v. 18, n. 1, p.142-148, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf?s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture. MenosPiper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf?s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were fr... Mostrar Tudo |
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Palavras-Chave: |
Callogenesis; Reguladores de crescimento. |
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Thesagro: |
Calogênese; Piperaceae. |
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Thesaurus Nal: |
growth regulators. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143083/1/2016-RBPM-Piper-permucronatum.pdf
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Marc: |
LEADER 02392naa a2200253 a 4500
001 2045154
005 2016-05-17
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, M. R. A. dos
245 $aInduction and growth pattern of callus from Piper permucronatum leaves.$h[electronic resource]
260 $c2016
520 $aPiper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf?s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture.
650 $agrowth regulators
650 $aCalogênese
650 $aPiperaceae
653 $aCallogenesis
653 $aReguladores de crescimento
700 1 $aGUIMARÃES, M. C. M.
700 1 $aPAZ, E. S.
700 1 $aMAGALHÃES, G. M. O.
700 1 $aSOUZA, C. A.
700 1 $aSMOZINSKI, C. V.
700 1 $aNOGUEIRA, W. O.
773 $tRevista Brasileira de Plantas Medicinais, Botucatu$gv. 18, n. 1, p.142-148, 2016.
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Embrapa Rondônia (CPAF-RO) |
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| 1. |  | FONSECA, L. M. F.; REIS, D. P.; GUIEIRO, C. S. M.; RIBAS, R. N. R.; MATTOS, B. B.; OLIVEIRA-PAIVA, C. A.; MARRIEL, I. E. Assessment of arginase and urease enzymes in rhizosphere of maize plants (zea mays l.) inoculated with Azospirillum diazotrophic bacteria. In: SIMPÓSIO INTERNACIONAL DE MICROBIOLOGIA E BIOTECNOLOGIA, 2., 2013, Viçosa, MG. Resumos. Viçosa, MG: UFV, 2013. SIMB 2013.| Tipo: Resumo em Anais de Congresso |
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